Use of Diagnostic Ions for the Detection of Fentanyl Analogs in Human Matrices by LC–QTOF

• DOI: 10.1021/jasms.1c00267
• Publication Date: 2021-11-18T17:32:56Z
• Authors: Kenneth D. Swanson; Rebecca L. Shaner; Logan C. Krajewski; William A. Bragg; Rudolph C. Johnson; Elizabeth I. Hamelin
• Keywords: Fentanyl; Ions; 03 medical and health sciences; 0302 clinical medicine; Synthetic Drugs; Tandem Mass Spectrometry; Humans; 01 natural sciences; 6. Clean water; Chromatography, Liquid; 0104 chemical sciences

To combat the ongoing opioid epidemic, our laboratory has developed and evaluated an approach to detect fentanyl analogs in urine plasma by screening LC–QTOF MS/MS spectra for ions that are diagnostic of core structure. data from a training set 142 were used select four product six neutral losses together provided most complete coverage (97.2%) compounds. Furthermore, using ion screen against 49 not resulted 95.9% those With this approach, lower reportable limits subset fentanyl-related compounds range 0.25 2.5 ng/mL 0.5 5.0 plasma. This innovative processing method was applied evaluate simulated exposure samples remifentanil carfentanil water their metabolites acid norcarfentanil urine. flexible enables way emerging clinical samples.