NanoLC-timsTOF-Assisted Analysis of Glycated Albumin in Diabetes-Affected Plasma and Tears

• DOI: 10.1021/jasms.3c00331
• Publication Date: 2023-11-28T20:21:05Z
• Authors: Yanqi Tan; Eduardo De La Toba; Stanislav S. Ruba...; Leanne T. Labriola; Christine Canfield; Dipanjan Pan; Jonathan V. Sweedler
• Keywords: Glycation End Products, Advanced; Glycated Hemoglobin; Blood Glucose; Lysine; Diabetes Mellitus; Humans; Glycated Serum Albumin; Serum Albumin; Biomarkers

Glycation is a spontaneous and nonenzymatic glycosylation. Glycated albumin (GA), which serves as an important biomarker in plasma the diagnosis characterization of diabetes, can be passively filtered from to tears. Tears are targets for research clinical diagnostics due ability collect this biofluid noninvasively repeatably. Therefore, analysis GA tear film provides information monitoring diabetes progression independent blood pathologies. Due limited volume (1–5 μL) natural film, we developed small assay using nano liquid chromatography-trapped ion mobility spectrometry-time-of-flight MS (nanoLC-timsTOF) platform glycated human films affected by diabetes. The peptides containing lysine 525, main glycation site GA, were relatively quantified represented level. results measurements showed that levels significantly higher diabetes-affected tears compared controls with p-value < 0.01. A strong correlation was observed samples (Pearson coefficient 0.92 0.0012). Moreover, number sites comparatively controls. Among all albumin, level 136/137 had more commonly used suggesting 136 /137 alternative plasma. Overall, our findings demonstrate progression, highlighting new possibilities quick noninvasive detection monitoring.