Determination of the Hydroperoxil Group Level in Polyethylene Glycols Using a Novel Mimic Enzyme Fe(III)-Mn(II)(HNAPTS)2

• DOI: 10.1021/jf020180r
• Publication Date: 2003-03-19T06:56:45Z
• Authors: Bo Tang; Guanwei Cui; Ming Du; Zhenzhen Chen; Yan Wang; Hongjian Wang
• Keywords: Binding Sites; Ascorbic Acid; Hydrogen Peroxide; Ferric Compounds; 01 natural sciences; Catalysis; Polyethylene Glycols; 0104 chemical sciences; Organometallic Compounds; Oxidation-Reduction; Horseradish Peroxidase; Schiff Bases

Two kinds of Schiff base metal complexes 2-hydroxy-1-naphthaldehyde-2-amino-5-phenylthiazole (HANPTS), Mn(II)(HNAPTS)2 and Fe(III)(HNAPTS)2, were synthesized used to mimic the active group horseradish peroxidase (HRP). The catalytic characteristics enzymes in oxidation reaction ascorbic acid (AsA) with OOH polyethylene glycols (PEGs) have been studied by a spectrophotometric method. Fe(III) has remarkable coordinated catalysis Mn(II)(HNAPTS)2; as result, ability Fe(III)-Mn(II)(HNAPTS)2 is 75% that HRP. possible mechanism was discussed. linear relationship between ΔA265AsA concentrations range 1.5 × 10-6 9.0 10-4 mol/L. proposed method successfully applied determination level different molecular weight PEGs. Keywords: Mimic enzyme; spectrophotometry; glycols;