Effects of Extraction Solvent Mixtures on Antioxidant Activity Evaluation and Their Extraction Capacity and Selectivity for Free Phenolic Compounds in Barley (HordeumvulgareL.)

• DOI: 10.1021/jf061087w
• Publication Date: 2006-08-18T10:01:50Z
• Authors: Haifeng Zhao; Jianjun Dong; Jian Lu; Jian Chen; Yin Li; Lianju Shan; Yan Lin; Wei Fan; Guoxian Gu
• Keywords: ABTS; Vanillic acid; Syringic acid

Four kinds of solvent extracts from three Chinese barley varieties (Ken-3, KA4B, and Gan-3) were used to examine the effects extraction mixtures on antioxidant activity evaluation their capacity selectivity for free phenolic compounds in through radical scavenging activity, reducing power metal chelating individual total contents. Results showed that had significant impacts estimation, as well different barley. The highest DPPH• ABTS•+ activities found 80% acetone extracts, whereas strongest •OH O2•- ethanol, methanol, water respectively. Additionally, (+)-catechin ferulic, caffeic, vanillic, p-coumaric acids, methanol (−)-epicatechin syringic acid, protocatechuic gallic acids. Furthermore, correlations analysis revealed TPC, power, positively correlated with each other (p < 0.01). Thus, routine screening higher was recommended extract or could be assess activity. Keywords: Barley; activity; solvent; compounds; TPC