Isolation and Identification of Strawberry Phenolics with Antioxidant and Human Cancer Cell Antiproliferative Properties

• DOI: 10.1021/jf071989c
• Publication Date: 2008-01-23T06:00:52Z
• Authors: Yanjun Zhang; Navindra P. Seeram; Rupo Lee; Lydia Feng; David Heber
• Keywords: Isolation; Identification; Cancer cell lines

Studies suggest that consumption of berry fruits, including strawberries ( Fragaria x ananassa Duch.), may have beneficial effects against oxidative stress mediated diseases such as cancer. Berries contain multiple phenolic compounds, which are thought to contribute their biological properties. Comprehensive profiling phenolics from was previously reported using high-performance liquid chromatography with mass spectrometry (HPLC-MS) detection. The current study reports the isolation and structural characterization 10 compounds strawberry extracts a combination Amberlite XAD16-resin C18 columns, HPLC-UV, nuclear magnetic resonance (NMR) spectroscopy methods. were cyanidin-3-glucoside 1), pelargonidin (2), pelargonidin-3-glucoside (3), pelargonidin-3-rutinoside (4), kaempferol (5), quercetin (6), kaempferol-3-(6'-coumaroyl)glucoside) (7), 3,4,5-trihydroxyphenyl-acrylic acid (8), glucose ester E)- p-coumaric (9), ellagic . Strawberry crude purified 1- evaluated for antioxidant human cancer cell antiproliferative activities by Trolox equivalent capacity (TEAC) luminescent ATP viability assays, respectively. Among pure anthocyanins 1 (7156 microM Trolox/mg), 2 (4922 4 (5514 Trolox/mg) most potent antioxidants. Crude (250 microg/mL) (100 inhibited growth oral (CAL-27, KB), colon (HT29, HCT-116), prostate (LNCaP, DU145) cells different sensitivities observed between lines. This adds growing body data supporting bioactivities fruit potential impact on health.