Studies on the Chemical Transformation of 20(S)-Protopanaxatriol (PPT)-Type Ginsenosides Re, Rg2, and Rf Using Rapid Resolution Liquid Chromatography Coupled with Quadruple-Time-of-Flight Mass Spectrometry (RRLC-Q-TOF-MS)

• DOI: 10.1021/jf302638f
• Publication Date: 2012-09-20T01:36:35Z
• Authors: Wei Wu; Qiujie Qin; Yingying Guo; Jinghui Sun; Shuying Liu
• Keywords: Chemical transformation; Protopanaxadiol; Aglycone

A rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) method was developed for analysis of chemical transformation 20(S)-protopanaxatriol (PPT)-type ginsenosides Re, Rg2, and Rf in acidic conditions. The products were identified by comparing the retention time standard compounds, accurate measurement, fragment ions obtained from RRLC-Q-TOF-tandem (MS/MS) analyses. specific product aglycone PPT (m/z 475), C-24- C-25-hydrated 493), Δ20(21) or Δ20(22) dehydration 457) MS/MS discussed structural characterization. Experiments demonstrated that mechanisms 20(S)-PPT-type conditions include hydrolysis saccharide substitution, dehydration, hydration addition reactions at C-24 C-25. pathway summarized. RRLC-Q-TOF-MS also applied comparative 20(S)-PPT ginsenoside related ginseng products.