Optimization of Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry for Detecting Methoxyphenolic Compounds in Pu-erh Tea

• DOI: 10.1021/jf304470k
• Publication Date: 2012-12-26T14:06:53Z
• Authors: Liping Du; Chao Wang; Jianxun Li; Dongguang Xiao; Changwen Li; Yongquan Xu
• Keywords: Solid-Phase Microextraction; Repeatability; Polydimethylsiloxane

A method based on headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry was developed for the analysis of volatile methoxyphenolic compounds in pu-erh tea. Six fibers different polarities were initially evaluated. The 75 μm carboxen/polydimethylsiloxane fiber exhibited highest extraction efficiency and selected further optimization. Plackett-Burman design used to screen brewing proportion tea water, amount tea, ionic strength, time, temperature, desorption rate agitation, equilibrium time. Box-Behnken then applied optimize significant factors. Under optimal conditions, proposed affords a wide range linearity, high linear regression coefficients (0.996-0.999), less than 9.0% repeatability relative standard deviation, limits detection ranging from 2.31 21.80 ng/g. has satisfactory accuracy, recoveries 79.08-113.9%. This successfully samples.