Anti-inflammatory Activity of Guluronate Oligosaccharides Obtained by Oxidative Degradation from Alginate in Lipopolysaccharide-Activated Murine Macrophage RAW 264.7 Cells

• DOI: 10.1021/jf503548a
• Publication Date: 2014-12-08T12:51:10Z
• Authors: Rui Zhou; Xuyang Shi; Yan Gao; Nan Cai; Zedong Jiang; Xu Xu
• Keywords: Lipopolysaccharides; 0301 basic medicine; 0303 health sciences; Alginates; Hexuronic Acids; Macrophages; Anti-Inflammatory Agents; NF-kappa B; Oligosaccharides; Nitric Oxide; Cell Line; Toll-Like Receptor 4; Mice; 03 medical and health sciences; Glucuronic Acid; Dietary Supplements; Animals; Mitogen-Activated Protein Kinases; Oxidation-Reduction

Alginate has notably diverse pharmacological activities. The present study investigated the anti-inflammatory activity of guluronate oligosaccharides prepared by oxidative degradation (GOS-OD) from alginate. GOS-OD significantly attenuated production nitric oxide (NO), prostaglandin E2 (PGE2), and reactive oxygen species (ROS), expression inducible synthase (iNOS) cyclooxygenase (COX)-2, secretion pro-inflammatory cytokines in lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cells. Moreover, potently decreased binding LPS to cell surface LPS-induced Toll-like receptor 4 (TLR4) cluster differentiation (CD) 14 expression. Additionally, could remarkably inhibit activation nuclear factor (NF)-κB mitogen-activated protein (MAP) kinase pathways These results indicate that may reduce LPS-stimulated inflammatory responses through blocking NF-κB MAP kinases, suggesting be considered as a potential nutraceutical for inflammation.