Efficient Sugar Release by the Cellulose Solvent-Based Lignocellulose Fractionation Technology and Enzymatic Cellulose Hydrolysis

• DOI: 10.1021/jf801303f
• Publication Date: 2008-08-15T07:01:26Z
• Authors: Geoffrey Moxley; Zhiguang Zhu; Y.-H. Percival Zhang
• Keywords: Enzymatic Hydrolysis; Beta-glucosidase

Efficient liberation of fermentable soluble sugars from lignocellulosic biomass waste not only decreases solid handling but also produces value-added biofuels and biobased products. Industrial hemp, a special economic crop, is cultivated for its high-quality fibers high-value seed oil, hollow stalk cords (hurds) are cellulosic waste. The cellulose-solvent-based lignocellulose fractionation (CSLF) technology has been developed to separate components under modest reaction conditions (Zhang, Y.-H. P.; Ding, S.-Y.; Mielenz, J. R.; Elander, Laser, M.; Himmel, McMillan, D.; Lynd, L. R. Biotechnol. Bioeng. 2007, 97 (2), 214- 223). Three pretreatment (acid concentration, temperature, time) were investigated treat industrial hemp hurds maximal sugar release: combinatorial result retention cellulose enzymatic hydrolysis. At the best treatment condition (84.0% H3PO4 at 50 degrees C 60 min), glucan digestibility was 96% hour 24 cellulase loading 15 filter paper units per gram glucan. scanning electron microscopic images presented CSLF-pretreated first time, suggesting that CSLF can completely destruct plant cell-wall structure, in good agreement with highest fastest hydrolysis rate. It found phosphoric acid above critical concentration (83%) sufficient time efficiently disrupt recalcitrant structures.