Method for Fabricating Supported Bilayer Lipid Membranes on Gold

• DOI: 10.1021/la000468f
• Publication Date: 2002-07-26T06:04:23Z
• Authors: Joydeep Lahiri; Peter Kalal; Anthony G. Frutos; Steven J. Jonas; Robert Schaeffler
• Keywords: Moiety; Self-assembled monolayer

The paper describes the synthesis of surfaces based on self-assembled monolayers (SAMs) alkanethiolates gold that were designed to support adsorption bilayer lipid membranes, and feasibility using these for making high-density arrays (HDAs) membranes. involved (i) formation SAMs 16-mercaptohexadecanoic acid (MHA) gold; (ii) activation carboxylic groups interchain anhydrides; (iii) treatment reactive with Brij-76 (C18H37(OCH2CH2)n-10OH) (1) or Brij-76-amine (2) (C18H37(OCH2CH2)n-10NH2); (iv) supported membranes by incubation printing lipids. oligo(ethylene glycol) moiety anchor provides a hydrophilic spacer between surface adsorbed enable incorporation membrane-spanning proteins extramembrane domains. Data from infrared spectroscopy confirmed coupling 2 through peptide bonds. Ellipsometric measurements showed an increase ∼15 Å in thickness SAM after 2; this observation suggests ∼25% MHA-SAM are derivatized Brij groups. yields 1 ∼40% observed 2. Experiments plasmon resonance (SPR) consistent binding bilayers Brij-derivatized surface, although alternative structures lipids cannot be ruled out; contrast, hexadecanethiolate bind monolayers. Biospecific neutravidin was incorporating biotinylated HDAs made onto quill-pin printer. Fluorescence microscopy indicated printed remained confined areas; observations demonstrate applicability commercial HDA printers generating membrane Brij-modified surfaces.