Nature-Inspired Creation of Protein−Polysaccharide Conjugate and Its Subsequent Assembly onto a Patterned Surface

• DOI: 10.1021/la0347096
• Publication Date: 2003-10-21T09:59:34Z
• Authors: Tianhong Chen; David A. Small; Li-Qun Wu; Gary W. Rubloff; Reza Ghodssi; Rafael Vazquez-Du...; William E. Bentley; Gregory F. Payne
• Keywords: Conjugate

A protein's functional properties can be adjusted by conjugating it to other polymers. We used a nature-inspired route create protein−polysaccharide conjugate and examined the of this conjugate. Specifically, enzyme tyrosinase was oxidize accessible tyrosine residues model protein green fluorescent (GFP). Oxidation yields quinone that are "activated" for covalent conjugation GFP nucleophilic groups aminopolysaccharide chitosan. Conjugation chitosan conferred distinct GFP. The GFP−chitosan observed have pH-responsive, "smart" properties, could conjugated onto gel matrix. Additionally, selectively deposited micropatterned surface in response an applied voltage. This method provides simple safe proteins chitosan, these conjugates readily assembled patterned surfaces.