Surface Investigation on Biomimetic Materials to Control Cell Adhesion: The Case of RGD Conjugation on PCL

• DOI: 10.1021/la100207q
• Publication Date: 2010-03-29T14:31:12Z
• Authors: Filippo Causa; Edmondo Battista; Raffaella Della M...; Daniela Guarnieri; Maria Iannone; Paolo A. Netti
• Keywords: Surface Modification; Penetration (warfare); Aminolysis

The cell recognition of bioactive ligands immobilized on polymeric surfaces is strongly dependent ligand presentation at the cell/material interface. While small peptide sequences such as Arg-Gly-Asp (RGD) are being widely used to obtain biomimetic interfaces, surface characteristics after immobilization well receptors deserve more detailed investigation. Here, we an RGD-based sequence poly(epsilon-caprolactone) (PCL), a largely widespread material in biomedical applications, polymer aminolysis. along with efficacy functionalization was monitored by analysis (FTIR-ATR, contact angle measurements, free energy determination) and spectrophotometric assays specially adapted for analytical quantification functional groups and/or peptides Particular attention paid evaluation number, morphology, penetration depth engrafted substrates. In particular, typical morphology distribution evidenced raised from crystallites, while significant molecules revealed. NIH3T3 fibroblast adhesion studies verified correct enhanced attachment conjugation. Such work proposes morphological approach characterization study treatment