Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

• DOI: 10.1021/la303032f
• Publication Date: 2012-12-04T20:46:44Z
• Authors: Yu Niu; Ana I. Matos; Luisa M. Abrantes; Ana S. Viana; Gang Jin
• Keywords: Protein Adsorption; Protein G; Surface Modification

Carbon disulfide (CS2) can spontaneously react with amine groups to form dithiocarbamates on gold surface, providing the possibility immobilize some compounds primary or secondary in one step. Using this principle, an immunosensor interface prepared for immunoglobulin G (IgG) sensing surface toward anti-IgG has been fabricated first time by simply immersing slides into a mixed aqueous solution of CS2 and protein A, followed incubation solution. The reaction between A UV–vis spectroscopy, whereas cyclic voltammetry employed characterization modified both methods indicating that immobilization is implemented CS2. Conventional ellipsometry, atomic force microscopy (AFM), as well plasmon resonance (SPR) have used evaluate specific binding IgG anti-IgG, revealing specifically captured biosensing interface, maintaining its bioactivity. Compared direct adsorption constructed far more sensitive capture target molecule. In addition, proven good capability inhibit nonspecific adsorption, supported control experiments using lysozyme BSA. To conclude, antibody one-step method potential simple convenient modification approach development.