Expression of MUC5AC in Ocular Surface Epithelial Cells Using Cationized Gelatin Nanoparticles

• DOI: 10.1021/mp200155t
• Publication Date: 2011-07-19T15:55:31Z
• Authors: Giovanni Konat Zorzi; Laura Contreras-Ruiz; Jenny Evelin Párraga; Antonio López-García; Rafael Romero Bello; Yolanda Diebold; Begoña Seijo; Alejandro Sánchez
• Keywords: Gelatin; Zeta potential

Decreased production of the mucin MUC5AC in eye is related to several pathological conditions, including dry syndrome. A specific strategy for increasing ocular levels not yet available. Using a plasmid specially designed encode human MUC5AC, we evaluated ability hybrid cationized gelatin nanoparticles (NPs) containing polyanions (chondroitin sulfate or dextran sulfate) transfect epithelial cells. NPs were developed using ionic gelation technique and characterized by small size (<200 nm), positive zeta potential (+20/+30 mV), high association efficiency (>95%). mRNA protein detected conjunctival cells after vitro transfection NPs. The vivo administration resulted significantly higher expression conjunctiva compared untreated control naked plasmid. These results provide proof-of-concept that these are effective vehicles gene therapy candidates restoring concentration surface.