Efficient Delivery of Sticky siRNA and Potent Gene Silencing in a Prostate Cancer Model Using a Generation 5 Triethanolamine-Core PAMAM Dendrimer

• DOI: 10.1021/mp2006104
• Publication Date: 2011-12-30T16:19:22Z
• Authors: Xiaoxuan Liu; Cheng Liu; Erik Laurini; Paola Posocco; Sabrina Pricl; Fanqi Qu; Palma Rocchi; Ling Peng
• Keywords: Triethanolamine

Successful achievement of RNA interference in therapeutic applications requires safe and efficient vectors for siRNA delivery. In the present study, we demonstrate that a triethanolamine (TEA)-core PAMAM dendrimer generation 5 (G5) is able to deliver sticky siRNAs bearing complementary An/Tn 3′-overhangs effectively prostate cancer model vitro vivo produce potent gene silencing heat shock protein 27, leading notable anticancer effect. The (n = or 7) overhangs characteristic these molecules help self-assemble into "gene-like" longer double-stranded RNAs thus endowing low such as G5 with greater delivery capacity. addition, act protruding molecular arms allow molecule enwrap promote better interaction stronger binding, ultimately contributing toward improved activity G5. Consequently, combination therapeutics may constitute promising silencing-based approach combating castration-resistant tumors other cancers diseases, which no effective treatment currently exists.