Composite Organic−Inorganic Nanoparticles as Raman Labels for Tissue Analysis

• DOI: 10.1021/nl062453t
• Publication Date: 2007-01-17T14:02:14Z
• Authors: Lei Sun; Kung-Bin Sung; Claire Dentinger; Barry Lutz; Lienchi Nguyen; Jingwu Zhang; Haoyu Qin; Mineo Yamakawa; Manqiu Cao; Yu Lu; AJ Chmura; Jing Zhu; Xing Su; Andrew A. Berlin; Selena Chan; Beatrice Knudsen
• Keywords: Male; Keratin-18; Histocytochemistry; Prostate; Enzyme-Linked Immunosorbent Assay; Prostate-Specific Antigen; Spectrum Analysis, Raman; 01 natural sciences; Antibodies; 3. Good health; 0104 chemical sciences; Humans; Nanoparticles; Nanotechnology; Protein Binding

Composite organic-inorganic nanoparticles (COINs) are novel optical labels for detection of biomolecules. We have previously developed methods to encapsulate COINs and to functionalize them with antibodies. Here we report the first steps toward application of COINs to the detection of proteins in human tissues. Two analytes, PSA and CK18, are detected simultaneously using two different COINs in a direct binding assay, and two different COINs are shown to simultaneously label PSA in tissue samples.