Highly Sensitive Electrochemiluminescent Nanobiosensor for the Detection of Palytoxin
Conductometry
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
General Physics and Astronomy
Food Contamination
Biosensing Techniques
biosensor
01 natural sciences
Carbon nanotube
electrochemiluminescence
Cnidarian Venoms
antibody
Nanotechnology
General Materials Science
14. Life underwater
BIOSENSORS; PALYTOXIN; CARBON NANOTUBES; electrochemiluminescence; shellfish
European Commission
Antibody
Shellfish
FP7
Electrochemiluminescence
Palytoxin
Acrylamides
EC
carbon nanotubes
SP1-Cooperation
General Engineering
Equipment Design
Biosensor; Palytoxin; Carbon nanotubes; Antibody; Electrochemiluminescence; Shellfish
0104 chemical sciences
Equipment Failure Analysis
shellfish
palytoxin
Health
Luminescent Measurements
Biosensor
Food Analysis
DOI:
10.1021/nn302573c
Publication Date:
2012-08-22T19:07:48Z
AUTHORS (15)
ABSTRACT
Marine toxins appear to be increasing in many areas of the world. An emerging problem in the Mediterranean Sea is represented by palytoxin (PlTX), one of the most potent marine toxins, frequently detected in seafood. Due to the high potential for human toxicity of PlTX, there is a strong and urgent need for sensitive methods toward its detection and quantification. We have developed an ultrasensitive electrochemiluminescence-based sensor for the detection of PlTX, taking advantage of the specificity provided by anti-PlTX antibodies, the good conductive properties of carbon nanotubes, and the excellent sensitivity achieved by a luminescence-based transducer. The sensor was able to produce a concentration-dependent light signal, allowing PlTX quantification in mussels, with a limit of quantification (LOQ = 2.2 μg/kg of mussel meat) more than 2 orders of magnitude more sensitive than that of the commonly used detection techniques, such as LC-MS/MS.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (30)
CITATIONS (103)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....