Two-Dimensional Microscale Engineering of Protein-Based Nanoparticles for Cell Guidance

• DOI: 10.1021/nn400907f
• Publication Date: 2013-05-24T22:07:05Z
• Authors: Witold I. Tatkiewicz; Joaquin Seras-Fra...; Elena García-Fruitós; Esther Vazquez; Nora Ventosa; Karl Peebo; Imma Ratera; Antonio Villaverde; Jaume Veciana
• Keywords: Inclusion Bodies; 0301 basic medicine; Mice; 03 medical and health sciences; Tissue Engineering; NIH 3T3 Cells; Animals; Humans; Microtechnology; Nanoparticles; Proteins; Fibroblasts

Cell responses, such as positioning, morphological changes, proliferation, and apoptosis, are the result of complex chemical, topographical, and biological stimuli. Here we show the macroscopic responses of cells when nanoscale profiles made with inclusion bodies (IBs) are used for the 2D engineering of biological interfaces at the microscale. A deep statistical data treatment of fibroblasts cultivated on supports patterned with green fluorescent protein and human basic fibroblast growth factor-derived IBs demonstrates that these cells preferentially adhere to the IB areas and align and elongate according to specific patterns. These findings prove the potential of surface patterning with functional IBs as protein-based nanomaterials for tissue engineering.