A Cell-Penetrating Ratiometric Nanoprobe for Intracellular Chloride

• DOI: 10.1021/ol300086w
• Publication Date: 2012-05-25T19:16:20Z
• Authors: Luca Baù; Francesco Selvestrel; Maria Arduini; Ilaria Zamparo; Claudia Lodovichi; Fabrizio Mancin
• Keywords: Nanoprobe

NanoChlor, a nanoparticle-based fluorescent probe for chloride that is both ratiometric and capable of spontaneously penetrating neuronal cells at submillimolar concentrations, was designed studied. NanoChlor built on silica nanoparticles grafted with 6-methoxyquinolinium as the chloride-sensitive component fluorescein reference dye. A Stern–Volmer constant 50 M–1 measured in Ringer's buffer pH 7.2, response to chemically induced currents recorded real time hippocampal cells.