Proteomic Profiling of Human Pleural Effusion Using Two-Dimensional Nano Liquid Chromatography Tandem Mass Spectrometry
Adult
Proteomics
0301 basic medicine
0303 health sciences
Lung Neoplasms
Proteome
Molecular Sequence Data
Adenocarcinoma
Middle Aged
Mass Spectrometry
Neoplasm Proteins
Pleural Effusion
03 medical and health sciences
Humans
Electrophoresis, Gel, Two-Dimensional
Aged
Chromatography, Liquid
DOI:
10.1021/pr049746c
Publication Date:
2005-08-08T07:46:44Z
AUTHORS (5)
ABSTRACT
Pleural effusion, an accumulation of pleural fluid, contains proteins originated from plasma filtrate and, especially when tissues are damaged, parenchyma interstitial spaces of lungs and/or other organs. This study details protein profiles in human pleural effusion from 43 lung adenocarcinoma patients by a two-dimensional nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (2D nano-HPLC-ESI-MS/MS) system. The experimental results revealed the identification of 1415 unique proteins from human pleural effusion. Among these 124 proteins identified with higher confidence levels, some proteins have not been reported in plasma and may represent proteins specifically present in pleural effusion. These proteins are valuable for mass identification of differentially expressed proteins involved in proteomics database and screening biomarker to further study in human lung adenocarcinoma. The significance of the use of proteomics analysis of human pleural fluid for the search of new lung cancer marker proteins, and for their simultaneous display and analysis in patients suffering from lung disorders has been examined.
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