Proteomic and Phosphoproteomic Analysis at Diapause Initiation in the Cotton Bollworm, Helicoverpa armigera

• DOI: 10.1021/pr100356t
• Publication Date: 2010-08-05T09:23:45Z
• Authors: Yu-Xuan Lu; Wei-Hua Xu
• Keywords: Hemolymph

Diapause is a period of developmental arrest that allows species to adapt unfavorable conditions. Many insect reduce metabolic activity and then enter diapause at certain stage in their life cycles. The cotton bollworm, Helicoverpa armigera, will be destined for pupal when larvae are reared under short daylengths low temperature. brain an important organ decision, some signaling molecules from the diapause-destined individuals released into hemolymph regulate diapause. In this study, we performed 2-D gel-based comparative proteomic phosphoproteomic analyses search differentially expressed proteins between nondiapause- brains. A total 79 23 phosphoproteins showed significant differences these two groups, 41 10 were identified by MALDI-TOF/TOF MS. Further, gene expression patterns diapause- nondiapause-destined brains confirmed RT-PCR or Western blot analysis. These act change, stress response, signal transduction pathways early initiation. Thus, may depress metabolism pupae lead arrest.