Quantitative Analysis of Serum IgG Galactosylation Assists Differential Diagnosis of Ovarian Cancer

• DOI: 10.1021/pr4003992
• Publication Date: 2013-07-15T14:37:13Z
• Authors: Yifan Qian; Yisheng Wang; Xingwang Zhang; Lei Zhou; Zejian, Zhang; Jiejie Xu; Yuanyuan Ruan; Shifang Ren; Congjian Xu; Jianxin Gu
• Keywords: Adult; Ovarian Neoplasms; 0303 health sciences; Glycosylation; Molecular Sequence Data; Galactose; Middle Aged; 3. Good health; Diagnosis, Differential; 03 medical and health sciences; Carbohydrate Sequence; ROC Curve; Area Under Curve; CA-125 Antigen; Case-Control Studies; Immunoglobulin G; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Biomarkers, Tumor; Carbohydrate Conformation; Humans; Female; Protein Processing, Post-Translational

CA-125, the most frequently used biomarker for ovarian cancer detection, cannot provide accurate diagnosis due to its poor specificity as it may also increase in many benign gynecological conditions. Thus, reducing false-positive outcomes is urgently needed. Decrease terminal galactosylated N-glycans of serum IgG has been found various malignancies compared healthy controls. Here, this alteration galactosylation was extended be investigated between and conditions with similar elevated CA-125 levels, an attempt effectively distinguish subjects patients. In study 58 patients levels (>35 U/mL), degree measured from relative intensities digalactosyl (G2), monogalactosyl (G1), agalactosylated (G0) according formula G0/(G1 + G2·2). This ratio significantly higher malignant group than (0.74 vs 0.34; p < 0.0001). ROC analysis demonstrated improved 65.2% (by test alone) 84.6%, while maintaining sensitivity at 90% by incorporating quantitative current assay. The results suggest that combining generate overall more robust approach differential cancer.