Abundance-Ratio-Based Semiquantitative Analysis of Site-Specific N-Linked Glycopeptides Present in the Plasma of Hepatocellular Carcinoma Patients

• DOI: 10.1021/pr4011519
• Publication Date: 2014-03-17T10:45:53Z
• Authors: Hyoung-Joo Lee; Hyun-Jeong Cha; Jong-Sun Lim; Sun Hee Lee; Si Young Song; Hoguen Kim; William S. Hancock; Jong Shin Yoo; Young-Ki Paik
• Keywords: Fucosylation; Haptoglobin

Aberrant structures of site-specific N-linked glycans are closely associated with the tumorigenesis hepatocellular carcinoma (HCC), one most common fatal cancers worldwide. Vitronectin (VTN) is considered a candidate glycobiomarker HCC. In this study, we describe reliable and simple quantification strategy based on abundance ratios glycopeptides VTN to screen for potential biomarkers. A total 14 unique corresponding 27 were characterized at three sites (Asn-86, -169, -242) present in VTN. These could be good markers Among these glycans, ratio two representative glycoforms (fucosyl vs non-fucosyl) was significantly increased HCC plasma relative normal plasma. This also successfully applied another biomarker, haptoglobin. Furthermore, demonstrate that our approach employing tandem mass tag (TMT) target useful tool quantifying specific Our represents potentially screening platform discovery cancer-specific glycobiomarkers.