Abundance-Ratio-Based Semiquantitative Analysis of Site-Specific N-Linked Glycopeptides Present in the Plasma of Hepatocellular Carcinoma Patients
Fucosylation
Haptoglobin
DOI:
10.1021/pr4011519
Publication Date:
2014-03-17T10:45:53Z
AUTHORS (9)
ABSTRACT
Aberrant structures of site-specific N-linked glycans are closely associated with the tumorigenesis hepatocellular carcinoma (HCC), one most common fatal cancers worldwide. Vitronectin (VTN) is considered a candidate glycobiomarker HCC. In this study, we describe reliable and simple quantification strategy based on abundance ratios glycopeptides VTN to screen for potential biomarkers. A total 14 unique corresponding 27 were characterized at three sites (Asn-86, -169, -242) present in VTN. These could be good markers Among these glycans, ratio two representative glycoforms (fucosyl vs non-fucosyl) was significantly increased HCC plasma relative normal plasma. This also successfully applied another biomarker, haptoglobin. Furthermore, demonstrate that our approach employing tandem mass tag (TMT) target useful tool quantifying specific Our represents potentially screening platform discovery cancer-specific glycobiomarkers.
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