Targeted Glycoproteomic Identification of Biomarkers for Human Breast Carcinoma

• DOI: 10.1021/pr700792g
• Publication Date: 2008-02-14T06:01:23Z
• Authors: Karen L. Abbott; Kazuhiro Aoki; Jae-Min Lim; Mindy Porterfield; Rachelle Johnson; Ruth M. O’Regan; Lance Wells; Michael Tiemeyer; Michael Pierce
• Keywords: Breast carcinoma

Glycosylation is a dynamic post-translational modification that changes during the development and progression of various malignancies. During oncogenesis breast carcinoma, glycosyltransferase known as N-acetylglucosaminyltransferase Va (GnT-Va) transcript levels activity are increased due to activated oncogenic signaling pathways. Elevated GnT-V leads β(1,6)-branched N-linked glycan structures on glycoproteins can be measured using specific carbohydrate binding protein or lectin L-PHA. L-PHA does not bind nondiseased epithelial cells, but invasive cells show progressive increase in binding. We have developed procedure for intact L-PHA-affinity enrichment, followed by nanospray ionization mass spectrometry (NSI-MS/MS), identify potential biomarkers carcinoma. identified reactive from matched normal (nondiseased) malignant tissue isolated patients with ductal Comparison analysis data 34 proteins were enriched fractionation tumor relative at least 2 cases Of these proteins, 12 common all 4 analyzed. These results indicate enrichment strategies targeting particular change associated malignancy an effective method identifying