2-DE and LC-MS/MS for a Comparative Proteomic Analysis of BALf from Subjects with Different Subsets of Inflammatory Myopathies

• DOI: 10.1021/pr800943t
• Publication Date: 2009-03-20T18:15:47Z
• Authors: Ileana Passadore; Paolo Iadarola; Cristina Di Poto; Serena Giuliano; Carlomaurizio Mon...; Lorenzo Cavagna; Claudia Bonino; Federica Meloni; Anna Maria Fietta; Antonella Lisa; Roberta Salvini; Anna Maria Bardoni
• Keywords: Spots; Proteome

The protein profiles of bronchoalveolar lavage fluid (BALf) patients belonging to three selected subsets Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in expression related phenotype. From among 323+/-51 spots may represent most highly expressed proteins BALf these patients, 24 unique isolated identified. In particular, 9 present PM/DM only; 12 exclusive Overlap 3 AS patients. identified, few classified as cytoskeletal proteins, others involved oxidative stress number associated with general metabolic activity immunological response inflammation. This is first which evidence provided different are supports our contention proteomic approach would be beneficial discovering molecules could possible prognostic factors rare pathologies.