2-DE and LC-MS/MS for a Comparative Proteomic Analysis of BALf from Subjects with Different Subsets of Inflammatory Myopathies

• DOI: 10.1021/pr800943t
• Publication Date: 2009-03-20T18:15:47Z
• Authors: Ileana Passadore; Paolo Iadarola; Cristina Di Poto; Serena Giuliano; Carlomaurizio Mon...; Lorenzo Cavagna; Claudia Bonino; Federica Meloni; Anna Maria Fietta; Antonella Lisa; Roberta Salvini; Anna Maria Bardoni
• Keywords: Cofilin 1; Proteomics; Immunoblotting; PM/DM; Dermatomyositis; 576; 03 medical and health sciences; 0302 clinical medicine; Tandem Mass Spectrometry; Humans; Vimentin; Electrophoresis, Gel, Two-Dimensional; LC-MS/MS; Gelsolin; Aged; Proteins; Reproducibility of Results; 600; Middle Aged; 2-DE; Overlap Syndrome; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Bronchoalveolar Lavage Fluid; AS; Chromatography, Liquid

The protein profiles of bronchoalveolar lavage fluid (BALf) of patients belonging to three selected subsets of Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination of 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in protein expression profiles that were related to the phenotype. From among the 323+/-51 protein spots that may represent the most highly expressed proteins in BALf of these patients, 24 unique spots were isolated and proteins identified. In particular, 9 spots were present in BALf of PM/DM patients only; 12 spots were exclusive of Overlap patients and 3 spots of AS patients. From among the proteins identified, a few were classified as cytoskeletal proteins, others were involved in oxidative stress and a number of proteins were associated with general metabolic activity or immunological response and inflammation. This is the first study in which evidence is provided that a number of different proteins are expressed in different subsets of PM/DM and supports our contention that the proteomic approach would be beneficial in discovering molecules which could represent possible prognostic factors of these rare pathologies.