Effect of the enamel matrix derivative Emdogain® on the growth of periodontal pathogens in vitro

• DOI: 10.1034/j.1600-051x.2002.290110.x
• Publication Date: 2003-03-11T13:27:44Z
• Authors: Axel Spahr; Staale Petter Lyn...; Clemens Boeckh; Christer Andersson; Andreas Podbielski; Bernd Haller
• Keywords: Enamel matrix derivative; Prevotella intermedia; Actinobacillus; Bacterial growth; Agar diffusion test

Abstract Objectives: The aim of this study was to evaluate the effect Emdogain ® (EMD), used for periodontal regeneration, on growth pathogens like Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis and Prevotella intermedia. For comparison, we studied EMD several microbes associated with other oral diseases as well its non‐pathogenic inhabitants. Methods: Freshly prepared or vehicle propylene glycol alginate (PGA) alone were added calibrated suspensions microbes. As a control, imitating post‐surgical subgingival situation after flap closure, serum/NaCl‐solution mixture used. Aliquots assays taken at scheduled times calculation colony‐forming units cell densities over an observation period 24 h. Additionally, spotted onto selected, newly seeded growing agar plates see if inhibition zones could be produced. Results: revealed marked inhibitory gram‐negative pathogens. A. showed significant decrease ( p =0.012) in viable counts h when baseline. P. intermedia also reduction presence these cultures no detected anymore In contrast, observed gram‐positive bacteria. Conclusions: results suggest that has positive composition bacterial species wound, by selectively restricting periopathogens hamper wound healing reduce outcome regenerative procedures.