Immunoelectrophoretic Characterization of Proteolytic Enzymes

• DOI: 10.1038/1971012a0
• Publication Date: 2006-07-05T16:01:07Z
• Authors: V. RAUNIO; O. GABRIEL
• Keywords: Immunoelectrophoresis; Proteolytic enzymes; Agar gel; Immunodiffusion

THE detection of proteolytic activity after electro-phoresis in agar gel has been described previously1,2. It has been shown with a number of hydrolytic enzymes3 that the precipitation lines formed by the antigen–antibody reaction after immunoelectrophoresis in agar gel exhibit enzymatic activity. This enzymatic activity can be demonstrated qualitatively after washing and drying the slides in the usual way by utilizing conventional histochemical techniques.