In vitro Detection of Immunosuppressive Activity of Anti-lymphocyte Sera

• DOI: 10.1038/217658a0
• Publication Date: 2006-07-06T09:02:14Z
• Authors: J. F. BACH; B. ANTOINE
• Keywords: Immune Sera; Complement Fixation Tests; Antineoplastic Agents; Thymus Gland; In Vitro Techniques; Antibodies, Anti-Idiotypic; 3. Good health; Mice; Agglutination Tests; Animals; Lymph Nodes; Lymphocytes; Rabbits; Spleen

THE problem of in vitro evaluation of anti-lymphocyte sera (ALS) has not yet been solved. The tests used, such as leuco-agglutination, cytotoxicity and lymphocyte stimulation in culture, do not detect small amounts of anti-lymphocyte antibodies and, furthermore, the results of these tests are not regularly correlated with graft survival time1,2. We propose a new in vitro test, based on inhibition of an active function of the lymphocytes.