Myosin isoenzyme redistribution in chronic heart overload

• DOI: 10.1038/282105a0
• Publication Date: 2005-08-02T08:22:56Z
• Authors: Anne-Marie Lompre; Ketty Schwartz; Anne d'Albis; Gabrielle Lacombe; Nguyen Van Thiem; Bernard Swynghedauw
• Keywords: Adenosine Triphosphatases; Male; 0301 basic medicine; Myosin Subfragments; Cardiomegaly; Myosins; Adaptation, Physiological; Myocardial Contraction; Rats; Isoenzymes; Epitopes; 03 medical and health sciences; Animals

Since the first observation by Spann et al., it has become clear that in cardiac hypertrophy induced by a mechanical overloading, the velocity of shortening of the cardiac muscle (Vmax) is reduced (see ref. 2 for review). Most authors agree that this mechanical alteration is accompanied by a decrease in the Ca2+-dependent ATPase activity of myosin (see ref. 3 for review). The molecular basis of such changes was unknown because the structural modifications of the myosin molecule were ill-defined. Nevertheless, it has recently been shown that, like skeletal muscle myosin, cardiac myosin is composed of several polymorphic forms, comparable to isoenzymes. In the skeletal muscle, new functional requirements can induce changes in both contractile activity and type of myosin isoenzyme synthesised. We now report that an increase in cardiac work produced by mechanical overloading in rats induces the preferential synthesis of a cardiac myosin isoenzyme characterised by specific immunological and electrophoretic properties and exhibiting a lower ATPase activity. This adaptive change could account for the reduced shortening speed of this hypertrophied cardiac muscle.