Molecular cloning of a cDNA encoding human antihaemophilic factor

• DOI: 10.1038/312342a0
• Publication Date: 2004-11-18T02:00:36Z
• Authors: John J. Toole; John L. Knopf; John M. Wozney; Lisa A. Sultzman; Janet L. Buecker; Debra D. Pittman; Randal J. Kaufman; Eugene Brown; Charles Shoemaker; Elizabeth C. Orr; Godfrey W. Amphlett; W. Barry Foster; Mary Lou Coe; Gaylord J. Knutson; David N. Fass; Rodney M. Hewick
• Keywords: Cloning (programming); Sequence (biology); Ceruloplasmin

A complete copy of the mRNA sequences encoding human coagulation factor VIII:C has been cloned and expressed. The DNA sequence predicts a single chain precursor of 2,351 amino acids with a relative molecular mass (Mr) 267,039. The protein has an obvious domain structure, contains sequence repeats and is structurally related to factor V and ceruloplasmin.