Non-injection methods for the production of embryonic stem cell-embryo chimaeras

• DOI: 10.1038/365087a0
• Publication Date: 2003-08-12T00:37:03Z
• Authors: Stephen A. Wood; Nick D. Allen; Janet Rossant; Anna Auerbach; Andras Nagy
• Keywords: Embryology; 0303 health sciences; Microinjections; Chimera; Stem Cells; Embryo, Mammalian; Morula; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Blastocyst; Culture Techniques; Animals; Cells, Cultured; Cell Aggregation

The simple aggregation of pluripotent ES cells with morulae-stage embryos can result in viable chimaeras at a similar frequency to that of blastocyst injection. The savings in time and equipment inherent in the aggregation techniques, however, make this approach well worth considering.