Contaminated open fracture and crush injury: a murine model

• DOI: 10.1038/boneres.2014.50
• Publication Date: 2015-01-27T10:44:12Z
• Authors: Shawn R Gilbert; Justin Camara; Richard Camara; Lynn Duffy; Ken Waites; Hyunki Kim; Kurt Zinn
• Keywords: Vascularity; Crush injury; Animal model

Modern warfare has caused a large number of severe extremity injuries, many which become infected. In more recent conflicts, pattern co-infection with Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus emerged. We attempted to recreate this in an animal model evaluate the role vascularity contaminated open fractures. Historically, it been observed that infected bones frequently appear hypovascular, but association bone infection not examined models. Adult rats underwent femur fracture muscle crush injury followed by stabilization bacterial contamination A. complex aureus. Vascularity perfusion were assessed microCT angiography SPECT scanning, respectively, at 1, 2 4 weeks after injury. Quantitative cultures also obtained. Multi-bacterial infections successfully created, S. predominating. There was overall increase blood flow injured limbs markedly greater bacteria-inoculated limbs. Vessel volume group. Quadriceps atrophy seen both groups, model, fractures had than non-infected