socs7, a target gene of microRNA-145, regulates interferon-β induction through STAT3 nuclear translocation in bladder cancer cells

• DOI: 10.1038/cddis.2013.11
• Publication Date: 2013-02-07T15:05:13Z
• Authors: S Noguchi; N Yamada; M Kumazaki; Y Yasui; J Iwasaki; S Naito; Y Akao
• Keywords: Ectopic expression; TLR3

We recently reported that microRNA (miR)-145 is downregulated and induces apoptosis in human bladder cancer cells. Also, it suggested the ectopic expression of miR-145 with induction TRAIL several Here, we demonstrated a novel mechanism by Exogenous T24 NKB1 cells markedly increased levels interferon (IFN)-β, 2′–5′-oligoadenylate synthetase 1, which lies upstream 2′–5′ oligoadenylates/RNase L system, TRAIL, induced apparent caspase-dependent was suppressed cotreatment pan-caspase inhibitor; moreover, these were reduced an inhibitor. The did not depend on Toll-like receptor 3 (TLR3) expression, because TLR3-silencing failed to inhibit IFN-β miR-145. Then, focused suppressor cytokine signaling 7 (socs7), whose level upregulated compared its normal urothelial cells, as putative target gene involved Expectedly, exogenous decreased SOCS7, socs7-silencing enhanced transfection TLR3 ligand, polyinosinic acid-polycytidylic acid (PIC). results luciferase reporter assay revealed targeted socs7. In addition, significantly p-Akt growth Furthermore, or promoted nuclear translocation STAT3. conclusion, machinery through regulation SOCS7 closely associated apoptosis. Moreover, targeting socs7, resulted Additionally, our data indicate functioned oncogene, finding carcinogenesis