Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer

• DOI: 10.1038/cddis.2014.521
• Publication Date: 2014-12-11T13:54:48Z
• Authors: Y Feng; M Zhu; S Dangelmajer; Y M Lee; O Wijesekera; C X Castellanos; A Denduluri; K L Chaichana; Q Li; H Zhang; A Levchenko; H Guerrero-Cazares; A Quiñones-Hinojosa
• Keywords: Homing (biology); Regenerative Medicine

Abstract Adult human adipose-derived mesenchymal stem cells (hAMSCs) are multipotent cells, which abundant, easily collected, and bypass the ethical concerns that plague embryonic cells. Their utility accessibility have led to rapid development of clinical investigations explore their autologous allogeneic cellular-based regenerative potential, tissue preservation capabilities, anti-inflammatory properties, anticancer among others. hAMSCs typically cultured under ambient conditions with 21% oxygen. However, physiologically, exist in an environment much lower oxygen tension. Furthermore, standard shown limited proliferative migratory as well viability. This study investigated effects hypoxic culture on primary intraoperatively derived hAMSCs. hypoxia (hAMSCs-H) remained multipotent, capable differentiation into osteogenic, chondrogenic, adipogenic lineages. In addition, hAMSCs-H grew faster exhibited less cell death. had greater motility than normoxia-cultured homing ability glioblastoma (GBM) from brain tumor-initiating our patients vitro vivo. Importantly, did not transform tumor-associated fibroblasts were tumorigenic vivo . Rather, promoted cancer These findings suggest alternative culturing technique can enhance function hAMSCs, may be necessary for use treatment various pathologies including stroke, myocardial infarction, amyotrophic lateral sclerosis, GBM.