miR-216a inhibits osteosarcoma cell proliferation, invasion and metastasis by targeting CDK14

• DOI: 10.1038/cddis.2017.499
• Publication Date: 2017-10-12T12:43:02Z
• Authors: Quanbo Ji; Xiaojie Xu; Ling Li; Stuart B Goodman; Wenzhi Bi; Meng Xu; Yameng Xu; Zhongyi Fan; William J Maloney; Qinong Ye; Yan Wang
• Keywords: 0301 basic medicine; Mice, Inbred BALB C; Osteosarcoma; Binding Sites; Carcinogenesis; Transplantation, Heterologous; Bone Neoplasms; Kaplan-Meier Estimate; Cyclin-Dependent Kinases; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Animals; Humans; Original Article; Female; Neoplasm Invasiveness; Neoplasm Metastasis; 3' Untranslated Regions; Neoplasm Transplantation; Cell Proliferation

Abstract Osteosarcoma (OS) has emerged as the most common primary musculoskeletal malignant tumour affecting children and young adults. Cyclin-dependent kinases (CDKs) are closely associated with gene regulation in biology. Accumulating evidence indicates that aberrant function of CDK14 is involved a broad spectrum diseases clinical outcomes. MicroRNAs (miRNAs) crucial epigenetic regulators development OS. However, essential role molecular mechanisms by which miRNAs regulate oncogenesis progression OS have not been fully elucidated. Here we found expression was poor prognosis overall survival patients. Using dual-luciferase reporter assays, also miR-216a inhibits binding to 3′-untranslated region CDK14. Overexpression significantly suppressed cell proliferation, migration invasion vivo vitro inhibiting production. miR-216a-transfected cells effectively rescued suppression caused miR-216a. In addition, Kaplan–Meier analysis indicated predicted favourable outcomes for Moreover, downregulated patients negatively expression. Overall, these data highlight miR-216a/CDK14 axis novel pleiotropic modulator demonstrate mechanisms, thus suggesting intriguing possibility activation inhibition may be attractive therapeutic strategies treating