A new molecular mechanism underlying the EGCG-mediated autophagic modulation of AFP in HepG2 cells

• DOI: 10.1038/cddis.2017.563
• Publication Date: 2017-11-02T19:35:30Z
• Authors: Lin Zhao; Shengtang Liu; Jiaying Xu; Wei Li; Guangxin Duan; Haichao Wang; Huilin Yang; Zaixing Yang; Ruhong Zhou
• Keywords: Modulation (music)

Abstract Epigallocatechingallate (EGCG) is a major bioactive component of green tea and associated with health benefits against multiple diseases including cancer. As an indicator hepatocellular carcinoma (HCC), high levels α -fetal protein (AFP) are related to malignant differentiation poor prognosis cancer cells. In this study, EGCG can effectively reduce AFP secretion simultaneously induce aggregation in human HCC HepG 2 EGCG-stimulated autophagy induces the degradation aggregates Furthermore, we thoroughly studied underlying molecular mechanisms behind by using large-scale all-atom dynamics simulations, which revealed novel mechanism. directly interacts LC3-I protein, readily exposing pivotal Gly-120 site latter other important binding partners such as 1,2-distearoyl- sn -glycero-3-phosphoethanolamine promoting synthesis LC3-II, characteristic autophagosomal marker. Our results suggest that critical regulating modulating autophagic activities cells, providing basis for potentially preventing treating HCC.