Cytosine methylation of tRNA-Asp by DNMT2 has a role in translation of proteins containing poly-Asp sequences

• DOI: 10.1038/celldisc.2015.10
• Publication Date: 2015-06-09T08:07:16Z
• Authors: Raghuvaran Shanmugam; Jacob Fierer; Steffen Kaiser; Mark Helm; Tomasz P Jurkowski; Albert Jeltsch
• Keywords: Cytosine

Abstract The Dnmt2 RNA methyltransferase catalyses the methylation of C38 in anticodon loop tRNA-Asp, but molecular role this is unknown. Here, we report that mouse aspartyl-tRNA synthetase shows a four to fivefold preference for C38-methylated tRNA-Asp. Consistently, 30% reduced charging level tRNA-Asp was observed knockout (KO) murine embryonic fibroblast cells. Gene expression analysis with fluorescent reporter proteins fused an N-terminal poly-Asp sequence showed protein synthesis poly-Asp-tagged KO cells as well. same effect endogenous containing sequences, indicating Dnmt2-mediated regulates translation sequences. ontology searches sequences human proteome significant number these have roles transcriptional regulation and gene expression. Hence, exhibits post-transcriptional regulatory by controlling group target