Translation in astrocyte distal processes sets molecular heterogeneity at the gliovascular interface
[SDV] Life Sciences [q-bio]
0301 basic medicine
0303 health sciences
03 medical and health sciences
astrocyte
local translation
mRNAs localization
translating ribosome immunoprecipitation
gliovascular unit
endfeet
Article
DOI:
10.1038/celldisc.2017.5
Publication Date:
2017-03-28T12:59:11Z
AUTHORS (18)
ABSTRACT
AbstractAstrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate vascular functions as well as homeostasis at the vascular interface. To date, the astroglial mechanisms underlying these functions have been poorly addressed. Here we demonstrate that a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, a pool of messenger RNAs bound to ribosomes, the endfeetome, that primarily encodes for secreted and membrane proteins. We detected nascent protein synthesis in astrocyte endfeet. Finally, we determined the presence of smooth and rough endoplasmic reticulum and the Golgi apparatus in astrocyte perivascular processes and endfeet, suggesting for local maturation of membrane and secreted proteins. These results demonstrate for the first time that protein synthesis occurs in astrocyte perivascular distal processes that may sustain their structural and functional polarization at the vascular interface.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (72)
CITATIONS (142)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....