CD205-TLR9-IL-12 axis contributes to CpG-induced oversensitive liver injury in HBsAg transgenic mice by promoting the interaction of NKT cells with Kupffer cells

• DOI: 10.1038/cmi.2015.111
• Publication Date: 2016-04-04T08:42:24Z
• Authors: Xin Hou; Xiaolei Hao; Meijuan Zheng; Congfei Xu; Jun Wang; Rongbin Zhou; Zhigang Tian
• Keywords: TLR9; CpG Oligodeoxynucleotide; Kupffer cell

Gut-derived bacterial products contribute to liver inflammation and injury during chronic hepatitis B virus infection; however, the underlying mechanisms remain obscure. In this study, surface antigen transgenic (HBs-Tg) mice their wild-type (WT) control C57BL/6 were injected with CpG-oligodeoxynucleotides (ODNs) mimic translocation of gut microbial into systemic circulation. We found that, compared WT mice, HBs-Tg oversensitive CpG-ODN-induced injury, which was dependent on natural killer T (NKT) cells. CpG-ODN injection enhanced expression Fas ligand (FasL) NKT addition, hepatocytes from expressed higher levels than did those further augmented by CpG-ODN. Interaction FasL involved in cytotoxicity cells against mice. Moreover, Kupffer CD205 produced greater amounts interleukin (IL)-12 Finally, depletion cells, neutralization IL-12 or specific silencing significantly inhibited activation Our data suggest that CD205-expressing respond CpG-ODNs subsequently release promote cell activation. Activated induce damage through signaling pathway