TGF-β receptors in mouse ES-5 cells and their differentiated derivatives

• DOI: 10.1038/cr.1995.4
• Publication Date: 2012-03-20T12:13:49Z
• Authors: Weikang Shi; Jun Wu; Luxia Xu; Hsiaochien Tsung
• Keywords: Cell type

By radioreceptor binding studies with iodinated TGF-β1, it has been shown that an undifferentiated ES-5 cell expresses approximately 3270 receptors with a dissociation constant Kd=130pM, but after the induction of differentiation by retinoic acid and dBcAMP, the receptor number of a differentiated RA-ES-5 cell was increased about 80% and the Kd was also increased to 370 pM. Furthermore, more direct evidence supporting the expression of TGF-β type I and type II receptors in both ES-5 and RA-ES-5 cells has come from dot blot hybridization of cellular mRNA with cDNA probes for type I and type II receptors. Meanwhile, mRNA expression level of types I and II receptors in RA-ES-5 cells were higher than that in ES-5 cells. Down- regulation of TGF-β receptors with a significant decrease in the rate of cell proliferation in both cells, was found by employing a pretreatment with neutralizing antibody to TGF-β1. The possible role of receptors for TGF-β in cell differentiation is discussed here.