The mechanism of translation initiation on Aichivirus RNA mediated by a novel type of picornavirus IRES

• DOI: 10.1038/emboj.2011.306
• Publication Date: 2011-08-26T14:40:44Z
• Authors: Yingpu Yu; Trevor R Sweeney; Panagiota Kafasla; Richard J Jackson; Tatyana V Pestova; Christopher UT He...
• Keywords: Picornavirus; Eukaryotic translation; Picornaviridae; Nucleic acid structure

Picornavirus mRNAs contain IRESs that sustain their translation during infection, when host protein synthesis is shut off. The major classes of picornavirus IRESs (Types 1 and 2) have distinct structures and sequences, but initiation on both is determined by their specific interaction with eIF4G. We report here that Aichivirus (AV), a member of the Kobuvirus genus of Picornaviridae, contains an IRES that differs structurally from Type 1 and Type 2 IRESs. Its function similarly involves interaction with eIF4G, but its eIF4G-interacting domain is structurally distinct, although it contains an apical eIF4G-interacting motif similar to that in Type 2 IRESs. Like Type 1 and Type 2 IRESs, AV IRES function is enhanced by pyrimidine tract-binding protein (PTB), but the pattern of PTB's interaction with each of these IRESs is distinct. Unlike all known IRESs, the AV IRES is absolutely dependent on DHX29, a requirement imposed by sequestration of its initiation codon in a stable hairpin.