The effects of 30 days resveratrol supplementation on adipose tissue morphology and gene expression patterns in obese men

• DOI: 10.1038/ijo.2013.155
• Publication Date: 2013-08-20T07:35:20Z
• Authors: E Konings; S Timmers; M V Boekschoten; G H Goossens; J W Jocken; L A Afman; M Müller; P Schrauwen; E C Mariman; E E Blaak
• Keywords: Adult; Male; mice; 610; 612; resveratrol; METABOLISM; adipogenesis; insulin-resistance; 03 medical and health sciences; Double-Blind Method; Stilbenes; Adipocytes; Humans; Obesity; Enzyme Inhibitors; Wnt Signaling Pathway; Aged; 2. Zero hunger; INSULIN-RESISTANCE; 0303 health sciences; Adipogenesis; Cross-Over Studies; Receptors, Notch; Gene Expression Profiling; WOMEN; health; Radboudumc 3: Disorders of movement DCMN: Donders Center for Medical Neuroscience; Middle Aged; ADIPOGENESIS; adipose tissue; MICE; Treatment Outcome; Adipose Tissue; glucose-tolerance; Resveratrol; women; GLUCOSE-TOLERANCE; HEALTH; Radboudumc 0: Other Research RIHS: Radboud Institute for Health Sciences; metabolism; microarray; Signal Transduction

Polyphenolic compounds, such as resveratrol, have recently received widespread interest because of their ability to mimic effects of calorie restriction. The objective of the present study was to gain more insight into the effects of 30 days resveratrol supplementation on adipose tissue morphology and underlying processes. Eleven healthy obese men were supplemented with placebo and resveratrol for 30 days (150 mg per day), separated by a 4-week washout period in a double-blind randomized crossover design. A postprandial abdominal subcutaneous adipose tissue biopsy was collected to assess adipose tissue morphology and gene expression using microarray analysis. Resveratrol significantly decreased adipocyte size, with a shift toward a reduction in the proportion of large and very-large adipocytes and an increase in small adipocytes. Microarray analysis revealed downregulation of Wnt and Notch signaling pathways and upregulation of pathways involved in cell cycle regulation after resveratrol supplementation, suggesting enhanced adipogenesis. Furthermore, lysosomal/phagosomal pathway and transcription factor EB were upregulated reflecting an alternative pathway of lipid breakdown by autophagy. Further research is necessary to investigate whether resveratrol improves adipose tissue function.