Evaluation of three-dimensional biofilms on antibacterial bonding agents containing novel quaternary ammonium methacrylates

• DOI: 10.1038/ijos.2014.18
• Publication Date: 2014-04-11T09:28:42Z
• Authors: Han Zhou; Michael D Weir; Joseph M Antonucci; Gary E Schumacher; Xue-Dong Zhou; Hockin HK Xu
• Keywords: Quaternary Ammonium Compounds; 0301 basic medicine; 03 medical and health sciences; Microscopy, Confocal; Biofilms; Methacrylates; Original Article; Anti-Bacterial Agents

Antibacterial adhesives are promising to inhibit biofilms and secondary caries. The objectives of this study were synthesize incorporate quaternary ammonium methacrylates into adhesives, investigate the alkyl chain length effects on three-dimensional adherent for first time. Six with lengths 3, 6, 9, 12, 16 18 synthesized incorporated Scotchbond Multi-Purpose. Streptococcus mutans bacteria cultured resin form biofilms. Confocal laser scanning microscopy was used measure biofilm thickness, live/dead volumes live-bacteria percentage vs. distance from surface. Biofilm thickness greatest control; it decreased increasing length, reaching a minimum at 16. Live-biofilm volume had similar trend. Dead-biofilm increased length. adhesive 9 37% live near surface, but close 100% in top section. For 16, there nearly 0% throughout biofilm. In conclusion, strong antibacterial activity achieved by adding adhesive, live-biofilm decreasing as 3 typically only inhibited its surface; however, mostly dead entire is margins combat Dental resins incorporating agents could help destroy bacterial responsible tooth decay, new research shows. By forming surfaces such teeth, cells strengthen their chances survival. To avoid decay following restoration, important minimize growth mutans, commonly associated decay. Hockin Xu University Maryland co-workers across USA China investigated dental containing various salts. Their work focused survival different areas team found that most effective an salt carbon backbone This killed whole