Tissue-Type Plasminogen Activator Triggers the Synaptic Vesicle Cycle in Cerebral Cortical Neurons

• DOI: 10.1038/jcbfm.2015.155
• Publication Date: 2015-07-01T08:02:35Z
• Authors: Fang Wu; Enrique Torre; David Cuellar-Gir...; Lihong Cheng; g Yi; Edyta K Bichler; Paul S García; Manuel Yepes
• Keywords: Synapsin; Active zone

The active zone (AZ) is a thickening of the presynaptic membrane where exocytosis takes place. Chemical synapses contain neurotransmitter-loaded synaptic vesicles (SVs) that at rest are tethered away from release site, but after inflow Ca +2 elicited by an action potential translocate to AZ their neurotransmitter load. We report tissue-type plasminogen activator (tPA) stored outside cerebral cortical neurons, either intermixed with small clear-core or in direct contact membrane. found ischemia-induced neuronal tPA, treatment recombinant recruits cytoskeletal protein βII-spectrin and promotes binding SVs βII-spectrin, enlarging population proximity site. This effect does not require generation plasmin followed recruitment voltage gated calcium channels (VGCC) terminal leads -dependent synapsin I phosphorylation, freeing deliver Our studies indicate tPA activates SV cycle induces structural functional changes synapse required for successful neurotransmission.