The CD4+CD26− T-cell population in classical Hodgkin's lymphoma displays a distinctive regulatory T-cell profile

• DOI: 10.1038/labinvest.2008.24
• Publication Date: 2008-03-24T15:30:45Z
• Authors: Yue Ma; Lydia Visser; Tjasso Blokzijl; Geert Harms; Çiǧdem Atayar; Sibrand Poppema; Anke van den Berg
• Keywords: CYTOKINE GENE-EXPRESSION; Adult; CD4-Positive T-Lymphocytes; Male; IMMUNOLOGICAL SELF-TOLERANCE; Adolescent; HUMAN TH2 CELLS; Dipeptidyl Peptidase 4; Gene Expression; PERIPHERAL-BLOOD; 03 medical and health sciences; CC-CHEMOKINE; T-Lymphocyte Subsets; Humans; RNA, Messenger; REED-STERNBERG CELLS; Child; DIFFERENTIAL CHEMOKINE EXPRESSION; 0303 health sciences; RECEPTOR CCR4; Sclerosis; Staining and Labeling; Reverse Transcriptase Polymerase Chain Reaction; Ionomycin; Reproducibility of Results; Flow Cytometry; Hodgkin Disease; Immunohistochemistry; TRANSCRIPTION FACTORS; Female; Lymph Nodes; TUMOR MICROENVIRONMENT

Little is known about the gene expression profile and significance of rosetting CD4+CD26− T cells in classical Hodgkin's lymphoma (cHL). To characterize these cells, CD4+CD26+ T-cell populations were sorted from lymph node (LN) cell suspensions nodular sclerosis HL (NSHL) reactive LNs. mRNA profiles stimulated resting subsets evaluated with quantitative RT-PCR for 46 genes. We observed a higher percentage NSHL than The showed levels CD25, CTLA4, OX40 CCR4 compared LNs, supporting regulatory (Treg) type, this was validated by immunohistochemistry. Moreover, low or no Th1- Th2-related cytokines IL-2, IFN-γ, IL-13, IL-12B, IL-4, IL-5, chemoattractant receptor CRTH2. Besides Tregs, Th17 may exist based on significantly IL-17 level both NSHL. Upon stimulation vitro, lack upregulation most cytokine genes indicated an anergic character subset. Anergy fits Treg probably explaining immunosuppressive mechanism involved