The neutrophil-recruiting chemokine GCP-2/CXCL6 is expressed in cystic fibrosis airways and retains its functional properties after binding to extracellular DNA

• DOI: 10.1038/mi.2015.43
• Publication Date: 2015-05-20T14:38:00Z
• Authors: S Jovic; H M Linge; M M Shikhagaie; A I Olin; L Lannefors; J S Erjefält; M Mörgelin; A Egesten
• Keywords: Neutrophil Extracellular Traps; Neutrophil elastase

Infections in cystic fibrosis (CF), often involving Pseudomonas aeruginosa, result from a dysregulated airway immunity where one hallmark is the accumulation of necrotic and apoptotic immune cells, particular neutrophils. In addition, neutrophils actively release DNA, forming neutrophil extracellular traps (NETs) that contain antimicrobial proteins. Altogether, free DNA complex with actin accumulates lumen, resulting highly viscous sputum provides an anionic matrix, binding cationic this study, granulocyte chemotactic protein 2 (GCP-2)/CXCL6, neutrophil-activating chemokine bactericidal properties, was detected epithelium CF patients also present azurophilic specific granules Elastase neutrophils, but not P. completely degraded CXCL6 (chemokine (C-X-C motif) ligand 6). colocalized both sputa vitro-formed NETs. vitro, bound KD 2,500 nM. Interestingly, receptor-activating properties (against neutrophils) remained largely unaffected presence DNA. However, were reduced by Taken together, expressed CF, retaining its functional even after to scaffold CF.