Microsatellite analysis of the DCC gene in nephroblastomas: pathologic correlations and prognostic implications

• DOI: 10.1038/modpathol.3800022
• Publication Date: 2003-11-20T15:09:24Z
• Authors: Amsha Ramburan; Runjan Chetty; G P Hadley; Richard Naidoo; Dhirendra Govender
• Keywords: Microsatellite Instability; Histology

Microsatellite instability has been reported in a wide variety of cancer types. Inactivation or loss tumour suppressor genes shown to result cell cycle deregulation and neoplastic growth. We conducted microsatellite study using fluorescent-based DNA technology determine whether mutations the sequences deleted colorectal (DCC) gene, at 18q21.1, have any pathologic correlation prognostic significance nephroblastomas. Normal was isolated from 106 cases nephroblastoma standard proteinase K digestion phenol-chloroform extraction method paraffin wax-embedded tissue. Polymerase chain reaction three markers; D18S21, D18S34 D18S58, for DCC gene were performed. The polymerase products analysed on ALF Express Automated sequencer. results correlated with age diagnosis, preoperative chemotherapy, clinicopathological stage, histological classification patient outcome chi(2) test. Allelic imbalance/loss heterozygosity appeared be more frequent genetic aberration than 20% showing allelic only 9% instability. Genetic aberrations unfavourable histology tumours compared favourable (P=0.012). All patients one marker died independent stage (P=0.016). There no statistically significant difference when chemotherapy stage. In conclusion, this found that multiple involving locus may play role progression nephroblastomas, hence confer poorer prognosis.