A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1

• DOI: 10.1038/msb.2008.10
• Publication Date: 2008-03-04T18:15:14Z
• Authors: Véronique de Bera...; David Vallenet; Vanina Castelli; Marielle Besnard; Agnès Pinet; Corinne Cruaud; Sumitta Samair; Christophe Lechap...; Gabor Gyapay; Céline Richez; Maxime Durot; Annett Kreimeyer; François Le Fèvre; Vincent Schächter; Valérie Pezo; Volker Döring; Claude Scarpelli; Claudine Médigue; Georges N Cohen; Philippe Marlière; Marcel Salanoubat; Jean Weissenbach
• Keywords: 570; Medicine (General); Acinetobacter baylyi ADP1; QH301-705.5; [SDV.BBM]Life Sciences [q-bio]/Biochemistry; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Models, Biological; Article; 03 medical and health sciences; R5-920; Bacterial Proteins; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]; 616; Escherichia coli; essential genes; [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology; Biology (General); Molecular Biology; DNA Primers; Molecular Biology/Genomics [q-bio.GN]; 0303 health sciences; Acinetobacter; Models, Genetic; Systems Biology; Chromosome Mapping; systems biology; Gene Expression Regulation, Bacterial; Carbon; Culture Media; 3. Good health; growth phenotype; Mutation; Pseudomonas aeruginosa; deletion mutants; metabolism; Gene Deletion

We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. This essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes described as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies were undertaken to investigate ADP1 metabolism by (1) searching for discrepancies between our essentiality data and current metabolic knowledge, (2) comparing this essentiality data set to those from other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbon sources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a new resource for the study of gene function by forward and reverse genetic approaches and constitutes a robust experimental data source for systems biology approaches.