Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo

• DOI: 10.1038/ncomms10098
• Publication Date: 2015-12-07T11:22:02Z
• Authors: Duy T. Tran; Andrius Masedunskas; Roberto Weigert; Kelly G. Ten Hagen
• Keywords: 0301 basic medicine; 0303 health sciences; Secretory Vesicles; Cell Membrane; Cell Polarity; Myosins; Real-Time Polymerase Chain Reaction; Time-Lapse Imaging; Article; Actin-Related Protein 2-3 Complex; Actins; Exocytosis; Salivary Glands; Actin Cytoskeleton; 03 medical and health sciences; Imaging, Three-Dimensional; Animals; Drosophila Proteins; Drosophila; Wiskott-Aldrich Syndrome Protein

The actin cytoskeleton plays crucial roles in many cellular processes, including regulated secretion. However, the mechanisms controlling F-actin dynamics this process are largely unknown. Through 3D time-lapse imaging a secreting organ, we show that is actively disassembled along apical plasma membrane at site of secretory vesicle fusion and re-assembled directionally on membranes. Moreover, pore formation PIP2 redistribution precedes myosin recruitment to Finally, essential for branched nucleators Arp2/3- WASp cargo expulsion integration vesicular membranes with membrane. Our results highlight previously unknown exocytosis provide genetically tractable system image temporal spatial polarized secretion vivo.