Bacterial partition complexes segregate within the volume of the nucleoid

• DOI: 10.1038/ncomms12107
• Publication Date: 2016-07-05T11:21:09Z
• Authors: Antoine Le Gall; Diego I. Cattoni; Baptiste Guilhas; Céline Mathieu-De...; Laura Oudjedi; Jean-Bernard Fiche; Jérôme Rech; Sara Abrahamsson; Heath Murray; Jean-Yves Bouet; Marcelo Nollmann
• Keywords: Nucleoid

Precise and rapid DNA segregation is required for proper inheritance of genetic material. In most bacteria archaea, this process assured by a broadly conserved mitotic-like apparatus in which NTPase (ParA) displaces the partition complex. Competing observations models imply starkly different 3D localization patterns components machinery during segregation. Here we use super-resolution microscopies to localize each component with respect bacterial chromosome. We show that Par proteins locate within nucleoid volume reveal volumetric complexes requires ATPase DNA-binding activities ParA. Finally, find system highly correlate dense chromosomal regions. propose new mechanism provides scaffold guide complexes.